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. 2022 Apr 19;23(6):e54275. doi: 10.15252/embr.202154275

Figure EV5. Cell surface markers associated with poor prognosis and the design of CAR molecules.

Figure EV5

  • A
    Kaplan‐Meier analysis of the TCGA‐HCC (TCGA‐LIHC) cohorts based on the expression levels of SLC34A2, FBN2, FOLR1, and SLC39A10 in the cohort samples (n = 364, high expression in red, low expression in black, the number of patients were indicated in figures, Statistical significance was determined using a log‐rank test).
  • B, C
    Representative IHC staining of FAP and MUC1 in a normal liver (PHH), primary HCC (pHCC) and five MTK‐transduced iHCC tissues (iHCC1‐1, iHCC2‐1, iHCC3‐1, iHCC4‐1, and iHCC5‐1) that were derived from five different donors (PHH1~5). Scale bars, 20 μm.
  • D
    Design of the pWPXLD‐CAR vector. pWPXLD‐CAR contains an anti‐FAP/MUC1 single‐chain fragment variation (scFv), CD28 transmembrane and endodomain, TLR2 endodomain, CD3ζ domain, and EGFP.
  • E
    The transduction efficiency of pWPXLD‐CAR is shown. Data are presented as the mean ± SD. N‐numbers refer to biological replicates.