Fig. 1.
Primary effector and memory T cell responses to SARS-CoV-2 virus in K18-ACE2tg mice. K18-ACE2tg mice were IN infected with 100 PFU of the USA-WA1/2020 (WA strain) of SARS-CoV-2. (A) Following SARS-CoV-2 infection, virus titers were measured in lungs. Single-cell suspensions of lungs were stained with viability dye, followed by Kb/S525 (VNFNFNGL) tetramers in combination with anti-CD4, CD8, and CD44. (B and C) Frequencies and numbers of S525-specific CD8 T cells in the lungs among gated CD8 T cells are shown in FACS plots and graphs. Data represent four independent experiments. Asterisks indicate significance at *P < 0.05, ***P < 0.0005, and ****P < 0.00005; ns, not significant. Data in each graph indicate mean ± SEM.