Fig. 1.

Schemes for RNA construct and fragment screening workflow. (A) RNA motifs 1 and 2 and the barcode helix are shown in colors; structure cassette helices are gray. (B) RNA is probed using SHAPE in the presence or absence of a small-molecule fragment. (C) Chemical modifications corresponding to ligand-dependent structural information are read out by multiplexed MaP sequencing. (D) Fragment hits are identified as multiple, statistically significant differences in SHAPE reactivities.