Cbln4 expression is enriched in the EC. (A–C) Cbln4 mRNA expression revealed by single-molecule RNA-FISH in adult mouse. RNA-FISH assay on coronal hippocampus (A) and entorhinal cortical sections (B) and on horizontal brain sections of the entorhinal and hippocampal area (C). Boxed regions on the Left are enlarged at Right. (Abbreviations: HPF, hippocampal formation; fi, fimbria; LGv, ventral part of the lateral geniculate complex; CA, cornu ammonis; DG, dentate gyrus; PAR, parasubiculum; MB, midbrain; Vsub, ventral subiculum; V3, third ventricle; MH, medial habenula; LH, lateral habenula; PVT, paraventricular nucleus of the thalamus; PH, posterior hypothalamic nucleus; TH, thalamus; HY, hypothalamus; sm, stria medullaris; PAG, periaqueductal gray; Str, striatum, CB, cerebellum; OB, olfactory bulb).