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. 2022 May 11;119(20):e2123421119. doi: 10.1073/pnas.2123421119

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Copyright © 2022 the Author(s). Published by PNAS.

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Fig. 5. — Postsynaptic deletion of Neo1 in the DG selectively ablates LTP at EC→DG synapses. Whole-cell voltage-clamp recordings from dentate gyrus granule cells for stimulation of inputs from the medial entorhinal cortex through the MPP and lateral entorhinal cortex through the LPP from control (ΔCre) and dentate gyrus Neo1 cKO (Cre) mouse brain slices. (A) Summary graph (Left) and sample traces (Right) of PPRs show no significant difference between the two conditions. MPP: ΔCre 1.02 ± 0.07, Cre 0.87 ± 0.08; LPP: ΔCre 1.26 ± 0.04, Cre 1.21 ± 0.04. (B) Summary graph (Left) and sample traces (Right) of NMDAR–AMPAR ratios show no significant difference between the two conditions. MPP: ΔCre 0.34 ± 0.03, Cre 0.37 ± 0.07; LPP: ΔCre 0.32 ± 0.06, Cre 0.28 ± 0.02. (C and D) Summary graph (Left) and sample traces (Right) of input–output relationships of AMPAR EPSCs for incremental stimulation intensities show no significant difference between the two conditions. (E–J) LTP is blocked in both MPP and LPP following postsynaptic deletion of Neo1 in the dentate gyrus. (E) Sample EPSC traces before and after LTP induction (Top) and time course of LTP (Bottom) for stimulation of the MPP in control (ΔCre) and Neo1 cKO (Cre) mouse brain slices. (F) Cumulative distribution of normalized LTP, ΔCre 1.82 ± 0.14, n = 7. Cre 0.92 ± 0.06, n = 7, *P = 0.0001 and (G) normalized change in PPR, ΔCre = −0.17 ± 0.11, Cre −0.05 ± 0.07, 35 to 40 min following LTP induction. (H and I) Same as in E and F except for stimulation of the LPP. (H) Sample EPSC traces before and after LTP induction (Top) and time course of LTP (Bottom) for stimulation of the LPP in control (ΔCre) and Neo1 cKO (Cre) mouse brain slices. (I) Cumulative distribution of normalized LTP, ΔCre 1.58 ± 0.06, Cre 1.15 ± 0.7, *P = 0.001 and (J) normalized change in PPR, ΔCre −0.1 ± 0.04, Cre 0.003 ± 0.076. Data are presented as mean ± SEM, *P < 0.05 (two-tailed t test).