Figure 1.
Experimental design. In the first phase, the N-glycome of the adult female rat spinal cord was characterized. Phase II examined how this profile changes in response to spinal cord transection, and treatment with collagen hydrogel in either a random or aligned orientation, at 7 and 14 days post-injury (dpi). The third phase employed X. laevis to model injury and compared the glycosylation response in the regenerative premetamorphic stage and the nonregenerative postmetamorphic stage, at 1 and 7 dpi. UPLC, ultrahigh-performance liquid chromatography; WAX-HPLC, weak anion exchange high-performance liquid chromatography; LC-MS, liquid chromatography coupled mass spectrometry. Created with BioRender.com.
