Experimental
design. In the first phase, the N-glycome of the adult
female rat spinal cord was characterized. Phase II examined how this
profile changes in response to spinal cord transection, and treatment
with collagen hydrogel in either a random or aligned orientation,
at 7 and 14 days post-injury (dpi). The third phase employed X. laevis to model injury and compared the glycosylation
response in the regenerative premetamorphic stage and the nonregenerative
postmetamorphic stage, at 1 and 7 dpi. UPLC, ultrahigh-performance
liquid chromatography; WAX-HPLC, weak anion exchange high-performance
liquid chromatography; LC-MS, liquid chromatography coupled mass spectrometry.
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