Summary of the types of N-glycan identified in the healthy adult
rat spinal cord. (A) All of the major classes of N-glycan can be found
in the spinal cord, oligomannose, hybrid, and branched. Branched glycans
also occur with a bisecting GlcNAc β(1–4) linked to the
central mannose. Some more unusual features such as acetylated sialic
acids, sulfate groups, and α-linked galactose can also be found
on complex or hybrid glycans. (B) Number of branches on complex glycans,
some also feature a bisecting GlcNAc β(1–4) linked to
the central mannose. A2 and A3 structures are most common. (C) Charge
abundance as calculated using WAX-HPLC. (D) Extent of branch elongation
with galactose on each class of N-glycan. (E) Proportion of total
glycans decorated with both core and outer-arm fucose. (F) Distribution
of fucosylation across the main N-glycan classes.