FIG. 2.

PCR amplicon of 232 bp and PCR-RFLP types of 232-bp amplicons of different HGs of reference strains of Aeromonas spp. PCR amplicon and PCR-RFLP types of reference and wild-type strains of Aeromonas spp. on 4% Metaphor agarose gel (Bioconcept, Allschwil, Switzerland) stained with ethidium bromide at 0.5 μg/ml (Bio-Rad). Lanes: 1 and 15, DNA size marker VIII (Roche Diagnostics AG; catalog no. 1336 045); 2, a PCR-RFLP 2 of A17 (ATCC 7966), a reference strain of HG1 isolated from canned milk from the United States; 3, PCR-RFLP 2 of A307 (ATCC 51108), a reference strain of HG2 isolated from a fish in France; 4, PCR-RFLP 2 of A14 (LMG 13674), a reference strain of HG3 that is an environmental isolate from Germany; 5, PCR-RFLP type 1 of A914 (CDC 9179), a reference strain of HG6 (source unknown); 6, PCR-RFLP 1 of A312 (ATCC 43979), a reference strain of HG7 that is a fish isolate from France; 7, PCR-RFLP 1 of A10 (CCUG 30356), a reference strain of HG8 that is an environmental isolate from Germany; 8, PCR-RFLP 1 of A901 (ATCC 35624), a reference strain of HG10 that is a clinical isolate (sputum) from the United States; 9, PCR-RFLP 1 of A926 (CDC 3136), a reference strain of HG11 that is a water isolate from the United States; 10, PCR-RFLP 3 of A1645 (ATCC 49659), a reference strain of HG13 that is a clinical isolate from the United States; 11, uncut 232-bp amplicon of strain A1645; 12, PCR-RFLP 2 of a wild-type strain (BP 3) of HG1 that is a clinical isolate of HG1 from Bangladesh; 13, PCR-RFLP 2 of a wild-type strain (CBK 45) of HG3 that is a pork meat isolate from Switzerland; 14, PCR-RFLP 1 of a wild-type strain (BE 6) of HG8 that is an environmental isolate from Bangladesh.