TABLE 2.
Results of PCR and characteristics of the 232-bp amplicons of reference strains
| Organism | Reference no. | Source | Country of origin | PCRa | No. of PCR-RFLPb fragments | % Similarity by PCR-ASAc | Gene similarity |
|---|---|---|---|---|---|---|---|
| A. hydrophila (HG1) | |||||||
| A17d | LMG 13439 | Unknown | Germany | + | 3 | 96 | Hemolysin-enterotoxin |
| A19 | LMG 13440 | Unknown | Germany | − | |||
| A22 | Not available | Feces | Switzerland | − | |||
| A34 | LMG 13441 | Feces | Switzerland | − | |||
| A52 | LMG 13442 | Feces | Switzerland | − | |||
| A82 | LMG 13656 | Feces | Switzerland | − | |||
| A145 | LMG 13657 | Feces | Switzerland | + | 3 | 99 | Hemolysin-enterotoxin |
| A162 | LMG 13658 | Feces | Switzerland | − | |||
| A167 | LMG 13659 | Feces | Switzerland | + | 3 | 98 | Hemolysin-enterotoxin |
| A306 | ATCC 7966Te | Milk | United States | + | 3 | 97 | Hemolysin-enterotoxin |
| A. bestiarum (HG2) | |||||||
| A2 | LMG 13445 | Human | Germany | + | 3 | 92 | Hemolysin-enterotoxin |
| A13 | LMG 13446 | Environmental | Germany | − | |||
| A39 | LMG 13447 | Water | Switzerland | + | 3 | 91 | Hemolysin-enterotoxin |
| A169 | LMG 13448 | Feces | Switzerland | + | 3 | 94 | Hemolysin-enterotoxin |
| A307 | CDC 9533-76T | Fish | France | + | 3 | 94 | Hemolysin-enterotoxin |
| A908 | ATCC 14715 | Fish | France | + | 3 | 94 | Hemolysin-enterotoxin |
| A936 | LMG 13664 | Unknown | United States | + | 3 | 94 | Hemolysin-enterotoxin |
| A937 | LMG 13665 | Unknown | United States | + | 3 | 94 | Hemolysin-enterotoxin |
| A976 | LMG 13666 | Water | United States | + | 3 | 94 | Hemolysin-enterotoxin |
| A1613 | LMG 13667 | Water | United States | + | 3 | 95 | Hemolysin-enterotoxin |
| A. hydrophila (HG3) | |||||||
| A7 | LMG 13452 | Environmental | Germany | + | 3 | 91 | Hemolysin-enterotoxin |
| A8 | LMG 13453 | Environmental | Germany | + | 3 | 91 | Hemolysin-enterotoxin |
| A14 | LMG 13674 | Environmental | Germany | + | 3 | 91 | Hemolysin-enterotoxin |
| A15 | LMG 13675 | Environmental | Germany | + | 3 | 91 | Hemolysin-enterotoxin |
| A63 | LMG 13450 | Feces | Switzerland | + | 3 | 91 | Hemolysin-enterotoxin |
| A99 | LMG 13449 | Feces | Switzerland | + | 3 | 91 | Hemolysin-enterotoxin |
| A220 | LMG 13661 | Feces | Switzerland | + | 3 | 91 | Hemolysin-enterotoxin |
| A308 | CDC 043-84 | Water | France | + | 3 | 91 | Hemolysin-enterotoxin |
| A938 | Not available | Unknown | United States | + | 3 | 91 | Hemolysin-enterotoxin |
| A1422 | Not available | Unknown | United States | + | 3 | 91 | Hemolysin-enterotoxin |
| A. salmonicida (HG3) | |||||||
| Not available | ATCC 33658T | Fish | United Kingdom | + | 3 | 91 | Hemolysin-enterotoxin |
| Not available | ATCC 33659 | Fish | United Kingdom | − | |||
| A. caviae (HG4) | |||||||
| A1 | LMG 13454 | Human | Germany | − | |||
| A3 | Not available | Unknown | Germany | − | |||
| A309 | ATCC 15468T | Guinea pig | France | − | |||
| A. caviae (HG5A) | |||||||
| A6 | LMG 13460 | Environmental | Germany | − | |||
| A75 | LMG 13461 | Feces | Switzerland | − | |||
| A310 | ATCC 51107 | Fish | France | − | |||
| A. caviae (HG5B) | |||||||
| A117 | LMG 13465 | Feces | Switzerland | − | |||
| A137 | Not available | Feces | Switzerland | − | |||
| A213 | LMG 13466 | Feces | Switzerland | − | |||
| A. eucrenophila (HG6) | |||||||
| A311 | ATCC 23309 | Fish | Unknown | + | 2 | 76 | Hemolysin |
| A914 | CDC 9179 | Unknown | Unknown | + | 2 | 76 | Hemolysin |
| A. sobria (HG7) | |||||||
| A312 | ATCC 43979T | Fish | France | + | 2 | 76 | Hemolysin |
| A915 | LMG 13469 | Fish | United States | − | |||
| A. veronii biovar sobria (HG8) | |||||||
| A10 | LMG 13067 | Environmental | Germany | + | 2 | 76 | Hemolysin |
| A11 | LMG 13693 | Environmental | Germany | + | 2 | 78 | Hemolysin |
| A27 | LMG 13694 | Unknown | Switzerland | + | 2 | 78 | Hemolysin |
| A132 | Not available | Feces | Switzerland | + | 2 | 76 | Hemolysin |
| A. veronii biovar veronii (HG10) | |||||||
| A901 | ATCC 35624T | Human | United States | + | 2 | 77 | Hemolysin |
| A. jandaei (HG9) | |||||||
| A179 | LMG 13064 | Feces | Switzerland | − | |||
| A919 | LMG 13065 | Feces | United States | − | |||
| A1642 | ATCC 49568T | Feces | United States | − | |||
| A. encheleia (HG11) | |||||||
| A902 | ATCC 35941 | Human | New Zealand | − | |||
| A926 | LMG 13076 | Water | United States | + | 2 | 76 | Hemolysin |
| A1653 | LMG 13061 | Water | Germany | + | 3 | 99 | Hemolysin-enterotoxin |
| A. schubertii (HG12) | |||||||
| A903 | ATCC 43700T | Human | United States | − | |||
| A922 | LMG 13473 | Human | United States | − | |||
| A. trota (HG14) | |||||||
| A1645 | ATCC 49659 | Human | United States | + | 4 | 82 | Hemolysin |
| A1646 | ATCC 49657T | Feces | India | + | 4 | 82 | Hemolysin |
| A1647 | ATCC 49660 | Feces | Thailand | + | 4 | 82 | Hemolysin |
| Non-Aeromonas spp. | |||||||
| Bacillus cereus | ATCC 11778 | Unknown | United States | − | |||
| Bacteroides coagulans | ATCC 7050 | Milk | United Kingdom | − | |||
| Clostridium perfringens | ATCC 13124 | Cow | United Kingdom | − | |||
| C. perfringens | CIP 60-19 | Sheep | France | − | |||
| C. perfringens | CIP 60-60 | Dog | France | − | |||
| Candida albicans | ATCC 10231 | Unknown | United States | − | |||
| Escherichia coli | ATCC 25922 | Clinical | United States | − | |||
| Listeria monocytogenes | ATCC 7644 | Human | Unknown | − | |||
| Micrococcus luteus | ATCC 9341 | Soil | United States | − | |||
| Proteus vulgaris | ATCC 13315 | Unknown | United Kingdom | − | |||
| Pseudomonas aeruginosa | ATCC 27853 | Blood | United States | − | |||
| Salmonella typhimurium | ATCC 14028 | Bovine | United States | − | |||
| Staphylococcus aureus | ATCC 25923 | Clinical | United States | − | |||
| S. aureus | ATCC 33862 | Unknown | Unknown | − | |||
| S. epidermidis | ATCC 12228 | Unknown | United States | − | |||
| Streptococcus agalactiae | ATCC 12386 | Unknown | Unknown | − | |||
| Vibrio parahemolyticus | ATCC 17802 | Food | Japan | − | |||
| V. vulnificus | CIP 75.4 | Blood | United States | − | |||
| V. vulnificus | CIP 81.90 | Blood | France | − | |||
| Yersinia enterocolitica | ATCC 23715 | Blood | Unknown | − |
a
PCR positivity.
b
PCR-RFLP fragments obtained when using HpaII endonuclease. PCR-RFLP 1 has two fragments, PCR-RFLP 2 has three fragments, and PCR-RFLP has four fragments.
c
PCR-ASA indicating similarity to the cytolytic enterotoxin gene (AHCYTOEN, GenBank accession no. M84709). PCR-ASA 1 has 76 to 78% similarity, PCR-RFLP 2 has 82% similarity, and PCR-ASA 3 has 91 to 99% similarity to the AHCYTOEN gene.
d
Altwegg reference number.
e
T, type strain in HG.