Table 1.

Primers used in multiplex qPCR analysis of stool samples by STH1 and STH2

qPCR	Target	Primer	Sequence (5′–3′)	Size (bp)	Gene detected	Final conc. (nM)
STH1	Strongyloides stercoralis	Strongy-F	GAATTCCAAGTAAACGTAAGTCATTAGC	101	18S	100
		Strongy-R	TGCCTCTGGATATTGCTCAGTTC			100
		Strongy-P	FAM-ACACACCGG/ZEN/CCGTCGCTGC-IBFQ			50
STH1	Trichuris trichiura	Tri-F	TTGAAACGACTTGCTCATCAACTT	76	18S	100
		Tri-R	CTGATTCTCCGTTAACCGTTGTC			100
		Tri-P	CY5-CGATGGTAC/TAO/GCTACGTGCTTACCATGG-IBRQ			50
STH1	Ascaris lumbricoides	Asc-F	GTAATAGCAGTCGGCGGTTTCTT	87	Internal transcribed spacer 1	100
		Asc-R	GCCCAACATGCCACCTATTC			100
		Asc-P	HEX-TTGGCGGAC/ZEN/AATTGCATGCGAT-IBFQ			50
STH2	Ancylostoma spp.	Anc-F	CGGGAAGGTTGGGAGTATC	104	Internal transcribed spacer 1	100
		Anc-R	CGAACTTCGCACAGCAATC			100
	A. duodenale	Aduo-P	HEX-TCGTTAC + T + GGGTGACGG-IBFQ			50
	A. ceylanicum	Acey-P	FAM-CCGTTC + CTGGGTGGC-IBFQ			50
STH2	Necator americanus	Nec-F	CTGTTTGTCGAACGGTACTTGC	101	Internal transcribed spacer 2	100
		Nec-R	ATAACAGCGTGCACATGTTGC			100
		Nec-P	CY5-CTG+TA+CTA+CG+CAT+TGTATAC-IBRQ			50
STH1 Y STH 2	Phocine herpes virus	PhHV-F	GGGCGAATCACAGATTGAATC		gB gene	100
		PhHV-R	GCGGTTCCAAACGTACCAA			100
		PhHV-P	Cy5-TTTTTATGTGTCCGCCACCATCTGGATC-BHQ2			50