Fig. 5.

MED23 p.P394H mutation might cause epirubicin resistance by promoting DNA damage repair by affecting the p-ATM- γ-H2A.X- p-CHK2 pathway. a Stable SUM-159 and BT-549 cells expressing wild-type MED23 and the P394H mutation were treated with epirubicin (100 nM for 0,15,30 and 60 min) and then subjected to immunoblotting for various DNA damage markers. b–e Stable SUM-159 and BT-549 cells expressing wild-type MED23 or P394H were treated with or without 1 μM epirubicin for 4 h and then stained with anti-γ-H2A.X antibody (green). Cell nuclei were counterstained with DAPI (blue). Quantitative results of γ-H2A.X-positive cells (the number of foci > 15 per cell) are shown in C and D. ***p < 0.001, **p < 0.01. Scale bar, 5 μm