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. 2022 Jun 6;21:123. doi: 10.1186/s12943-022-01595-9

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Silencing circVAPA suppresses cell viability, induces apoptosis of SCLC cells, and inhibits cell cycle progression in vitro. a RT-qPCR analysis of circVAPA and VAPA mRNA expression in SCLC cells treated with the corresponding siRNA. SCR, siRNA with scrambled sequences; si-circVAPA 1# and si-circVAPA 2#, two siRNAs specifically against the junction site of circVAPA. b Cell viability was assessed by the CellTiter-Glo assay. c Colony formation assay (DMS273) and soft agar colony formation assay (H82) were used to assess cell survival in SCLC cells transfected with the indicated siRNAs. d The apoptosis rate was analyzed by flow cytometry after depleting circVAPA in SCLC cells. e Effects on cell cycle progression analyzed by flow cytometry after downregulation of circVAPA. f The expressions of apoptosis-related protein (cleaved-PARP) and cycle-related protein (p21) were detected in SCLC cells transfected with the indicated siRNA by Western blot. β-actin was used as an internal reference. C.PARP, cleaved PARP. (All data are presented as the mean ± SD, ns, no significance; *P < 0.05; **P < 0.01; ***P < 0.001 by two-tailed Student’s t-test). Three independent assays were performed in the above assays