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. 2022 Jun 1;42(22):4488–4504. doi: 10.1523/JNEUROSCI.1499-21.2022

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Figure 5. — Inactivation of BLA-BNST neurons during conditioning does not affect sustained freezing to a prolonged cue. A, Rats were conditioned as described previously (Fig. 1A). B, Rats were injected with either saline (N = 24; black circles) or CNO (N = 24; gray circles) before conditioning. There was a main effect of time (B; F_(4.46,205.2) = 104, p < 0.0001, two-way RM ANOVA) on freezing behavior during training and testing (C; F_{(3.489,160.5)} = 19.95, p < 0.0001, two-way RM ANOVA). There was no main effect of drug (F_(1,46) = 1.144, p = 0.2904, two-way RM ANOVA) nor interaction (F_(10,460) = 1.356, p = 0.1983, two-way RM ANOVA) during training or testing (main effect of drug, F_(1,46) = 0.7682, p = 0.3853, two-way RM ANOVA; main interaction, F_(10,460) = 1.245, p = 0.2596, two-way RM ANOVA). When animals were separated out by sex, BLA-BNST inhibition during prolonged cued fear training had only a main effect of time during both training (D; F_{(4.243,93.35)} = 57.8, p < 0.0001, two-way RM ANOVA) and testing (E; F_{(3.761,82.74)} = 13.48, p < 0.0001, two-way RM ANOVA) in males. No main effect of drug nor interaction between drug and time was observed during training (main effect of drug, F_(1,22) = 0.1057, p = 0.7482, two-way RM ANOVA; main interaction, F_(10,220) = 1.028, p = 0.4205, two-way RM ANOVA) nor testing (main effect of drug, F_(1,22) = 0.002366, p = 0.9616, two-way RM ANOVA; main interaction, F_(10,220) = 0.5052, p = 0.8854, two-way RM ANOVA) in males. Similar results were observed in females with only a main effect of time during both training (F; F_(3.82,99.32) = 53.38, p < 0.0001, two-way RM ANOVA) and testing (G; F_{(3.255,84.63)} = 9.072, p < 0.0001, two-way RM ANOVA) but no main effect of drug nor interaction during training (main effect of drug, F_(1,26) = 3.528, p = 0.0716, two-way RM ANOVA; main interaction, F_(10,260) = 0.7071, p = 0.7176, two-way RM ANOVA) or testing (main effect of drug, F_(1,26) = 2.010, p = 0.1682, two-way RM ANOVA; main interaction, F_(10,260) = 1.105, p = 0.3589, two-way RM ANOVA).