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. 2022 Jun 1;42(22):4488–4504. doi: 10.1523/JNEUROSCI.1499-21.2022

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Figure 9. — Inhibition of BLA-BNST neurons during prolonged cued fear training reduces freezing in metestrous females. A, Proestrous (N = 9) and metestrous (N = 19) female rats were injected with either saline (black) or CNO (gray) before training as described previously (Fig. 1A). BLA-BNST inactivation had no effect on freezing behavior in proestrous females (B; main interaction, F_(10,70) = 0.5992, p = 0.8093; main effect of time, F_{(3.120,21.84)} = 11.29, p < 0.0001; main effect of drug, F_(1,7) = 0.1793, p = 0.6847, two-way RM ANOVA) and testing sessions (C; main interaction, F_(10,70) = 0.7499, p = 0.6754; main effect of time, F_{(2.409,16.86)} = 3.528, p = 0.0455; main effect of drug, F_(1,7) = 0.8542, p = 0.3861, two-way RM ANOVA). D, However, in metestrous females, BLA-BNST inhibition significantly reduced freezing during training (main interaction, F_(10,170) = 1.817, p = 0.0609; main effect of time, F_{(4.085,69.44)} = 4.41 = 18.31, p < 0.0001; main effect of drug, F_(1,17) = 6.172, *p = 0.0237, two-way RM ANOVA). E, Interestingly, this significant impact on training did not affect metestrous female freezing behavior during testing (main interaction, F_(10,170) = 0.6373, p = 0.7804; main effect of time, F_{(23.547,60.3)} = 8.395, p < 0.0001; main effect of drug, F_(1,17) = 1.170, p = 0.2944, two-way RM ANOVA).