Fig. 1.

Optimal library preparation and sequencing. (A) Total DNA is extracted from thorax and legs and all 8 extracts are pooled (B) and assessed for DNA quality and quantity. DNA extract is aliquoted [A1-A4] and (C) high molecular weight [HMW] DNA is precipitated [H1-H4] and used for ONT library preparations. (D) Each library [L1-L4] is sequenced on one Oxford Nanopore MinION flow cell. High accuracy base caller transforms the raw nanopore data into long reads [LR]. (E) Supernatant [SN] containing low molecular weight DNA [LMW] can also be cleaned [L] and used for the preparation of libraries [S] for short read sequencing [SR]