Figure 4.

Interruption of CREB/HDAC1 interaction rescues MPTP-induced CREB inactivation. A, Detection of the association between HDAC1 and CREB in mice overexpressing GAL4 or GAL4 M1 by immunoprecipitation from three separate experiments. *The overexpressed GAL4 M1. B, C, Immunofluorescence and Western blot analysis for p-CREB and NURR1 (probed with #ab41917, Abcam) expression in dopaminergic neurons of mice overexpressing GAL4 or GAL4 M1. *A nonspecific band detected by the anti-NURR1 antibody. n = 3 or 4 per group. Scale bars, 100 μm. Quantification of the blots normalized to CREB (middle) or tubulin (right). Three-way ANOVA (Bonferroni's). D, The mouse midbrains from Saline/MPTP mice treated with TSA or its vehicle were subjected to immunoprecipitation. Detection of the association between HDAC1 and CREB. Representative results from three separate experiments. E, F, Immunofluorescence and Western blot analysis for p-CREB and NURR1 (probed with sc-991, Santa Cruz) expression in the indicated groups. Scale bars, 100 μm. Quantification of the blots in F normalized to CREB (middle) or tubulin (right). Two-way ANOVA (Tukey's): *p < 0.05, **p < 0.01 versus Saline group; ^#p < 0.05, ^##p < 0.01, ^§p< 0.05, ^§§p< 0.01, ns, not significant as indicated.