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. 2022 Jun 6;20:255. doi: 10.1186/s12967-022-03468-7

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Expression and prognostic value analysis of RAF1 in clinical specimens. A–B qRT-PCR and WB assay of RAF1. C Immunohistochemical staining for RAF1. D Kaplan–meier survival curve of of risk factor for lymphatic metastasis. E Kaplan–meier survival curve of of risk factor for RAF1 expression. L, lymphatic metastasis patients' tumor tissue; NL, non-lymphatic metastasis patients’ tumor tissue; N, Normal tissue adjacent to the carcinoma. **P < 0.01, ***P < 0.001