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. 2022 Jun 6;20:255. doi: 10.1186/s12967-022-03468-7

Fig. 6.

Fig. 6

RAF1 regulates tumor growth and lymphatic metastasis in vivo. A Fluorescence imaging of nude mice, red arrow indicates suspected lymphatic metastasis. B, C Luminescence area and photon flux analysis. D Anatomy of foot-pad primary tumors and metastatic lymph nodes in nude mice, red arrow indicates metastatic lymph nodes; E Measurements of primary tumors and lymph nodes volume. FH qRT-PCR and WB assay of RAF1 and lymphangiogenic cytokines. I Immunohistochemical staining for RAF1 and LYVE-1. Abbreviations: sh-RNA, short hairpin RNA; NC, negative control; lv, lentivirus; OE, overexpression. *P < 0.05, **P < 0.01, ***P < 0.001