Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jun 7;20:262. doi: 10.1186/s12967-022-03467-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 4 — Chronic stress downregulates the stability of the p53 protein by promoting p53 ubiquitination. A-B HCT116 and LoVo cells were treated with EPI followed by treatment with cycloheximide (CHX) for the indicated times. The intensity of p53 expression at each time point was quantified by densitometry and plotted against time. C-D Immunoblots of HCT116 and LoVo cells treated with Epi for 5 days and then incubated with or without MG132 for 6 h. E Ubiquitin assays of HCT116 and LoVo cells treated with Epi and MG132. F-G Immunoblot analysis revealed that P53 overexpression reverses the effects of EPI-induced promotion of EMT and CSCs in CRC cells. H–J Mammosphere culture analysis revealed that p53 overexpression reverses the effect of EPI on the promotion of CSCs in CRC cells (scale bar: 100 μm). K–N Transwell assays revealed that p53 overexpression reverses the effect of EPI on the promotion of migration in CRC cells. O-P Colony formation assays revealed that p53 overexpression reverses the effect of EPI on the promotion of proliferation in CRC cells. *P < 0.05, **P < 0.01, ***P < 0.001