Fig. 6.

TRIM2 downregulates the stability of the p53 protein by promoting p53 ubiquitination. A–D Immunoblots of HCT116 and LoVo cells treated with CHX for the indicated times. The intensity of p53 expression at each time point was quantified by densitometry and plotted against time. E Ubiquitin assays of HCT116 and LoVo cells transfected with Sh-TRIM2 followed by treatment with MG132. F Co-IP and Western blot (WB) analysis indicated that endogenous p53 and TRIM2 bind to each other. G-H Immunofluorescence staining was performed using anti-TRIM2 antibody and anti-p53 antibody. DAPI was used to stain the nucleus (scale bar: 10 μm). I-J Immunoprecipitation of TRIM2 constructs and p53 in 293 T cells. K-L Immunoprecipitation of p53 constructs and TRIM2 in 293 T cells. *P < 0.05, **P < 0.01, ***P < 0.001