Extended Data Fig. 4. Data analysis of the Pan-RGC Trans-Seq data.

a-d, UMAP plots of Pan-RGC Trans-Seq data show major neuronal markers, including (a) Snap25 and (b) Syt1 as pan-neuronal markers, (c) Slc17a6 (vGluT2) for excitatory neurons including three ESCs, and (d) Gad2 for inhibitory neurons including five ISCs. These markers defined neuronal populations and separated the clusters among three ESCs (Slc17a6-positive, c) and five ISCs (Gad2-positive, d). e-g, We queried previously identified marker genes expressed in SC neuronal subsets (f)^22,80. Additionally, several cell-cell adhesion molecules, such as Type II Cdhs (g) and neuropeptides and their putative receptors (e), appeared as good molecular markers for neuronal subsets in the mammalian nervous system^81,82. Notably, when we queried the gene expression of Ntsr1 in the Trans-Seq dataset, we found no Ntsr1 expression in any SC neuron cluster (e). Dot-plots as bioinformatics predictions of (e) Neuropeptides and receptors, and (g) Cdhs for SC subset expression.