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. Author manuscript; available in PMC: 2022 Jun 7.
Published in final edited form as: Nat Neurosci. 2022 May 6;25(5):659–674. doi: 10.1038/s41593-022-01068-8

Fig. 6. Etv1-positive neurons in the SO do not uptake mWmC from the retina and do not receive retinal monosynaptic inputs.

Fig. 6.

a, in situ hybridization of Etv1 (red, RNA-probe) showed no overlap with ESC1 neurons (green), even though the somata of both populations reside in the same sublamina (SO) at the SC. Scale bars: 100 μm. b, Genetically labeled Etv1-positive neurons (Etv1-CreER; LSL-YFP) were detected as a subset of neurons within the SO, above the vAChT-positive bands marking the SGI (red). Scale bar: 100 μm. The dotted line marks the pial surface. c Etv1-positive neurons were not labeled by mWmC following retinal injections of AAV2-WmC. In contrast, neighboring cells within the SO were labeled by mWmC. Red dotted circles mark cells that were not labeled by mWmC. Scale bar: 20um. d-e, Whole-cell recordings showed Etv1-positive SC neurons do not receive retinal inputs (e), while the ESC1s (Ntsr1-GN209-YFP) receive direct retinal inputs (d). The stimulation paradigms were established in Fig. 1. The blue lines indicate the onset of blue light to active ChR2.Postsynaptic currents persisted in TTX (1μM) and 4-AP (100μM). F, Average ESPC amplitudes in ESC1s (black) and Etv1-positive neurons (red). n= 5animals, ****, p<0.0001, two-sided Student’s t-test. Data in this figure are presented as mean ± SEM.