Fig. 6. Chemical structure of cyclic covalent biotinylated probe C-36 and western blotting analysis of HEK293T cell lysate labelling. (A) Chemical structure of thioether covalent biotinylated probe C-36. (B) SDS-PAGE/western blot analysis of HEK293T cell lysate and recombinant His6-MBP-PHD3 labelling (4.5 h at RT) using biotinylated thioether sulfonyl fluoride probe C-36.
