TABLE 2.

Mean absolute and relative changes in body weight and biochemical parameters among C57BL/6 mice after 4 months on HCD, by gender.

Parameters	HCD		Gender difference P-value
	Female	Male
	Mean ± SD (n)	Mean ± SD (n)
Body weight (BW, g)
T0 BW	19.42 ± 1.22(55)	22.22 ± 1.78(60)
T4 BW	32.40 ± 5.25(55)	43.86 ± 4.62(60)
ΔBW = T4-T0	12.98 ± 5.59(55)	21.64 ± 4.03(60)	< 0.0001****
CBW = ΔBW/T0	0.68 ± 0.31(55)	0.98 ± 0.19(60)	< 0.0001****
Total cholesterol (TC, mg/dL)
T0 TC	102.82 ± 14.28(55)	106.07 ± 20.16(59)
T4 TC	177.48 ± 39.96(54)	232.64 ± 58.38(59)
ΔTC = T4-T0	74.31 ± 34.55(54)	126.28 ± 54.34(58)	< 0.0001****
CTC = ΔTC/T0	0.72 ± 0.31(54)	1.23 ± 0.64(58)	< 0.0001****
High-density lipoprotein cholesterol (HDL, mg/dL)
T0 HDL	67.31 ± 11.88(55)	82.64 ± 23.77(59)
T4 HDL	102.67 ± 18.04(54)	130.61 ± 25.71(59)
ΔHDL = T4-T0	35.30 ± 24.11(54)	47.34 ± 40.63(58)	0.0615
CHDL = ΔHDL/T0	0.59 ± 0.45(54)	0.71 ± 0.65(58)	0.2618
Blood glucose (Glu, mg/dL)
T0 Glu	145.24 ± 29.57(55)	148.92 ± 33.43(59)
T4 Glu	162.67 ± 25.95(54)	229.02 ± 50.64(59)
ΔGlu = T4-T0	16.96 ± 37.78(54)	81.41 ± 55.45(58)	< 0.0001****
CGlu = ΔGlu/T0	0.18 ± 0.41(54)	0.63 ± 0.53(58)	< 0.0001****
Triglycerides (TG, mg/dL)
T0 TG	59.24 ± 17.07(55)	76.27 ± 24.86(59)
T4 TG	47.19 ± 10.41(54)	53.76 ± 17.65(59)
ΔTG = T4-T0	−12.26 ± 19.88(54)	−22.83 ± 29.85(58)	0.0307*
CTG = ΔTG/T0	−0.13 ± 0.36(54)	−0.19 ± 0.54(58)	0.4938

HCD, high-calorie diet; SD, standard deviation; n, number of animals; T0, before HCD feeding; T4, after 4 months on HCD. Δ, absolute change; C, relative change; P values were calculated using the unpaired two-tailed Student’s t-test. Asterisks indicate statistical significance.