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SDS-PAGE of purified ChiA and deletion mutants. Lane 1, molecular mass standards, namely, rabbit muscle phosphorylase b (94 kDa), bovine serum albumin (67 kDa), egg white ovalbumin (43 kDa), bovine erythrocyte carbonic anhydrase (30.1 kDa), and soybean trypsin inhibitor (20.1 kDa); lane 2, purified ChiA (131,235 Da); lane 3, purified ChiAΔ1 (97,553 Da); lane 4, purified ChiAΔ2 (70,567 Da); lane 5, purified ChiAΔ3 (58,871 Da); lane 6, purified ChiAΔ4 (33,832 Da).
