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. 2022 Jun 7;12(8):2007–2018. doi: 10.1007/s13346-022-01185-8

Fig. 6.

Fig. 6

HER2-NBs elicit an anti-HER2 T cell IFNγ response. Spc were cultured with D2F2/E2 cells at a 10:1 ratio for 48 h in an IFNγ ELISpot assay. In order to evaluate the CD8-restricted response, D2F2/E2 were incubated with the MHC-I blocking antibody. Specific spots were calculated by subtracting the spots produced by the spc in medium alone from the spots produced in the presence of tumor cells. * p < 0.05 to utd