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. 2022 May 24;9:787627. doi: 10.3389/fcvm.2022.787627

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Copyright © 2022 Lebert, Ravi, Kensah and Christoph.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison (to scale) of video data obtained with different cameras in calcium- and voltage-sensitive optical mapping experiments. All hearts were stained with voltage-sensitive fluorescent dye Di-4-ANEPPS. (A) Human iPSC-derived cardiomyocyte monolayer expressing the genetically encoded calcium indicator GCaMP-X imaged at 40 fps with 800 × 800 pixel resolution (0.74mm field of view) using an IDS μEye UI-3060CP-M-GL camera (refer to Supplementary Video 6). (B) Rabbit heart imaged at 447 fps with 320 × 500 pixel resolution using an IDS μEye UI-3060CP-M-GL camera (refer to Supplementary Video 3). (C) Rabbit heart imaged at 500 fps with 320 × 440 pixel resolution using a Basler acA720-520um camera (refer to Supplementary Videos 1, 2). (D) Rabbit heart imaged at 500 fps with 256 × 256 pixel resolution using a Brainvision Scimedia MiCAM N256 camera (refer to Supplementary Video 9). (E) Mouse heart imaged at 500 fps with 200 × 200 pixel resolution (cropped from original video imaged with 720 × 320 pixels) using a Basler acA720–520 um camera (refer to Supplementary Video 4). (F) Porcine heart imaged at 500 fps with 128 × 128 pixel resolution using a Teledyne Photometrics Evolve 128 camera. The video data in (A,F) can be processed in real-time, faster than the acquisition speeds at 40 and 500 fps, respectively, using GPU-accelerated processing, refer to Figures 8, 9.