TABLE 2.
Utilization of heterologous siderophores by P. putida N1R Pvd−
Test strain | Siderophore production phenotype | Cross-feeding of N1R Pvd−a |
---|---|---|
P. putida | ||
N1R | Pvd+ | + |
N1R Pvd− | Pvd− | − |
WCS358 | Pvd+ | + |
WCS358 Pvd− | Pvd− | − |
E. cloacae | ||
JL1157 | Ent+ Iuc+ | + |
LA122 | Ent+ Iuc− | + |
LA266 | Ent− Iuc+ | + |
LA235 | Ent− Iuc− | − |
The capacity of N1R Pvd− to utilize siderophores produced by test strains was detected in a cross-feeding assay. Molten KMB amended with 600 μg of 2,2′-dipyridyl per ml was seeded with 105 CFU of the indicator strain P. putida N1R Pvd− per ml. +, zone of growth surrounding colonies of test strains indicated siderophore cross-feeding; −, no detectable zone. The experiment was done three times with identical results.