Figure 6.

CA suppresses ER stress in IEC. Colitis was induced as described in Figure 1 and colon samples were collected on day 10. (A) Colonic sections were stained for BIP and DAPI. Representative immunofluorescence images are shown. (B) The expression levels of BIP, XBP1s, and CHOP were determined by qRT-PCR. (C) HCT-116 cells (an intestinal epithelial cell line) were incubated overnight with Thapsigargin (TG, 100 nM) in the presence or absence of CA (10 μM). The expression levels of BIP, CHOP, and XBP1s were determined by qRT-PCR. Data are presented as mean ± SD. Representative data of three independent experiments are shown. One-way analysis of variance (ANOVA) followed by Tukey's multiple comparisons test, *p < 0.05, **p < 0.01, and ***p < 0.001.