TABLE 1.

Growth, FD, and alcian blue staining of cells with respect to strain and culture conditions

Strain and culture conditions	Mean biomass dry wt (g) ± SDa	Mean growth rate (h−1) ± SD	Alcian blue stainingb	FDc
Wild type
Aerobiosis	14 ± 2d	0.44 ± 0.05	+++	4
Anaerobiosis	6.5 ± 1	0.37 ± 0.03	+	2
Anaerobiosis + antimycin	11 ± 1	0.44 ± 0.04	+	1
[rho0] mutant
Aerobiosis	11 ± 1.5	0.49 ± 0.05	+	2
Anaerobiosis	4.5 ± 1	0.37 ± 0.03	+	1

^aCells were harvested in the stationary growth phase. Results are expressed in lyophilized biomass harvested from a 1.5-liter fermentor. 

^bStaining was done as described in reference 1. 

^cFD was estimated as described in reference 11. 

^dAssays were performed in the culture medium and in Helm’s acetate buffer. The values shown are means of at least three determinations.