TABLE 2.
Coflocculation assays and aggregation by concanavalin A of untreated or antimycin-treated wild-type and [rho0] mutant S. cerevisiae NCYC 625
| Cells | Mean % of cells in suspension ± SD (FD)
|
|
|---|---|---|
| Coflocculationa | Aggregation by concanavalin Ab | |
| Controls | ||
| Wild type | 29 ± 4.3c (4) | 57 ± 4.4 (3) |
| [rho0] mutant | 65 ± 9.5 (2) | 82 ± 3.9 (1) |
| Antimycin-treated wild type | 90 ± 8.5 (1) | 93 ± 6.1 (0–1) |
| Mixtures | ||
| Untreated + antimycin-treated wild type | 63 ± 8.2 (2) | 74 ± 8.4 (2) |
| Wild type + mutant | 48 ± 5.3 (3) | 62 ± 6.6 (2) |
a
Cells were mixed at a 1:1 ratio in 100 mM succinate buffer (pH 4.0) for 4 to 6 h at 25°C.
b
Aggregation was started by addition of concanavalin A at 150 mg/liter.
c
The values shown are means of three determinations.