TABLE 4.
Yield and global chemical composition of PPM extractsa
| Parameter | Wild type
|
[rho0] mutant
|
|||
|---|---|---|---|---|---|
| wtA | wt N | wtAant | m A | m N | |
| Yieldb | 9.0 ± 0.12 | 11 ± 0.34 | 9.7 ± 0.24 | 10 ± 0.11 | 11 ± 0.15 |
| Carbohydratec | 42 ± 1.8 | 39 ± 2.5 | 45 ± 2.2 | 60 ± 4.1 | 40 ± 2.9 |
| Proteind | 16 ± 1.9 | 21 ± 1.7 | 19 ± 0.8 | 12 ± 1.3 | 22 ± 0.2 |
| Phosphatee | 4.3 ± 0.6 | 3.3 ± 0.4 | 2.3 ± 0.3 | 2.4 ± 0.2 | 2.8 ± 0.2 |
a
The results shown are means ± standard deviations. Carbohydrate, protein, and phosphate are expressed as percentages of lyophilized crude PPM extracts. Phosphate results are the means of three determinations. Yield; carbohydrate, and protein results are means of four determinations. The abbreviations are the same as in Table 3, except that N indicates anaerobiosis.
b
Values are percentages of lyophilized yeast cells.
c
Determined by the phenol-sulfuric acid method (6).
d
Determined by the 2,4-dinitrofluorobenzene method (10).
e
Determined by the ammonium molybdate-ascorbic acid reagent method (21).