Table 1.
The rhythm strength and period of locomotor activity under DD of flies expressing calcium channel RNAi transgenes in PDF neurons or all clock neurons
| Gene | CG no. | Genotype* | N † | AR, % | Period | pwr | Wid | SNR** | ACT day | ACT night | ACT cycle |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Control | — | pdf > dcr2 | 24 | 4 | 24.8 | 81.3 | 6.0 | 1.6 | 23.7 | 27.9 | 25.8 |
| tim > dcr2 | 14 | 21 | 24.2 | 66.3 | 5.1 | 1.6 | 20.4 | 10.2 | 15.3 | ||
| α1A cac | 43,368 | tim > JF02572 | 16 | 0 | 24.1 | 49.8 | 4.5 | 0.5 | 19.9 | 10.7 | 15.3 |
| pdf > JF02572 | 16 | 6 | 24.5 | 81.0 | 4.1 | 0.7 | 16.4 | 16.1 | 16.2 | ||
| pdf > kk104178 | 16 | 0 | 25.4 | 138.5 | 8.6 | 3.1 | 13.9 | 28.7 | 21.3 | ||
| tim > kk104178 | 14 | 7 | 24.4 | 41.1 | 4.9 | 1.0 | 14.8 | 21.0 | 17.9 | ||
| α1T | 15,899 | pdf > JF02150 | 14 | 7 | 24.5 | 75.1 | 4.9 | 1.5 | 22.1 | 15.6 | 18.8 |
| tim > JF02150 | 16 | 0 | 23.6 | 59.0 | 4.4 | 1.1 | 14.3 | 2.4 | 8.4 | ||
| pdf > KK100082 | 23 | 65 | 24.4 | 21.4 | 3.0 | 0.7 | 14.4 | 11.9 | 13.2 | ||
| tim > KK100082 | 23 | 83 | 24.2 | 13.2 | 2.0 | 0.4 | 13.5 | 12.5 | 13.0 | ||
| α1D | 4,894 | pdf > JF01848 | 14 | 0 | 24.3 | 66.7 | 4.4 | 0.9 | 18.7 | 13.4 | 16.1 |
| tim > JF01848 | 16 | 0 | 24.3 | 28.1 | 4.1 | 0.5 | 14.3 | 5.7 | 10.0 | ||
| pdf > GD1737 | 16 | 0 | 24.7 | 159.8 | 6.4 | 2.4 | 19.8 | 10.2 | 15.0 | ||
| tim > GD1737 | 23 | 0 | 24.4 | 170.8 | 6.3 | 2.3 | 18.4 | 14.8 | 16.6 | ||
| pdf > HMS00294 | 16 | 0 | 24.5 | 162.5 | 5.4 | 1.9 | 25.7 | 30.2 | 27.9 | ||
| tim > HMS00294 | 16 | 0 | 24.0 | 87.9 | 4.1 | 0.8 | 19.7 | 7.9 | 13.8 | ||
| α2δ | 12,295 | pdf > JF01825 | 16 | 6 | 24.3 | 50.8 | 4.6 | 0.7 | 27.1 | 18.0 | 22.6 |
| tim > JF01825 | 16 | 13 | 23.9 | 62.7 | 5.9 | 1.0 | 25.0 | 13.7 | 19.4 | ||
| pdf > KK101267 | 16 | 0 | 24.7 | 183.1 | 6.2 | 2.6 | 17.0 | 21.3 | 19.1 | ||
| tim > KK101267 | 15 | 0 | 24.0 | 115.5 | 4.9 | 1.4 | 20.7 | 11.8 | 16.2 | ||
| Orai | 11,430 | pdf > HMC03562 | 15 | 0 | 24.6 | 167.6 | 6.3 | 2.5 | 10.4 | 14.2 | 12.3 |
| tim > HMC03562 | 20 | 10 | 23.6 | 78.9 | 4.1 | 0.8 | 10.6 | 4.2 | 7.4 | ||
| pdf > uas-dOrai | 13 | 0 | 24.9 | 108.9 | 5.6 | 1.0 | 24.8 | 26.2 | 25.5 | ||
| tim > uas-dOrai | 15 | 0 | 24.3 | 115.4 | 5.7 | 1.2 | 16.1 | 8.8 | 12.4 | ||
| dSTIM | 9,126 | pdf > GLC01785 | 16 | 6 | 24.2 | 91.8 | 7.4 | 2.0 | 21.4 | 18.6 | 20.0 |
| tim > GLC01785 | 15 | 27 | 23.7 | 31.9 | 3.6 | 0.7 | 13.3 | 6.0 | 9.7 | ||
| pdf > KK102366 | 23 | 0 | 25.4 | 118.3 | 6.2 | 1.2 | 11.4 | 12.6 | 12.0 | ||
| tim > KK102366 | 22 | 5 | 23.9 | 105.5 | 4.8 | 1.6 | 11.9 | 12.6 | 12.2 | ||
| pdf > JF02567 | 16 | 0 | 24.5 | 113.5 | 5.3 | 1.3 | 23.5 | 24.5 | 24.0 | ||
| tim > JF02567 | 12 | 25 | 23.8 | 24.7 | 3.3 | 0.6 | 7.3 | 3.4 | 5.3 | ||
| pdf > uas-dSTIM | 15 | 0 | 24.3 | 129.4 | 5.8 | 1.2 | 23.6 | 11.5 | 17.5 | ||
| tim > uas-dSTIM | 14 | 0 | 23.7 | 137.1 | 5.3 | 1.4 | 21.1 | 6.2 | 13.7 | ||
| SERCA | 3,725 | pdf > JF01948 | 14 | 0 | 25.2 | 84.1 | 6.4 | 1.3 | 33.6 | 35.2 | 34.4 |
| tim > JF01948 | 12 | 50 | 25.3 | 12.7 | 2.8 | 1.4 | 13.1 | 10.1 | 11.6 | ||
| pdf > KK107371 | 16 | 13 | 24.5 | 40.9 | 4.4 | 0.7 | 28.3 | 29.1 | 28.7 | ||
| tim > KK107371 ‡ | 15 | 73 | 23.7 | 65.4 | 3.4 | 0.7 | 14.9 | 8.7 | 11.8 | ||
| RyR | 10,844 | pdf > HM05130 | 15 | 0 | 24.7 | 94.0 | 6.2 | 1.7 | 19.0 | 19.1 | 19.0 |
| tim > HM05130 | 16 | 6 | 24.0 | 49.8 | 4.6 | 0.8 | 17.4 | 6.7 | 12.0 | ||
| pdf > JF03381 | 39 | 28 | 24.4 | 55.2 | 3.4 | 0.7 | 16.5 | 11.7 | 14.1 | ||
| tim > JF03381 | 28 | 7 | 23.9 | 128.1 | 5.3 | 1.4 | 19.1 | 10.1 | 14.6 | ||
| pdf > KK101716 | 13 | 8 | 24.7 | 165.1 | 6.6 | 2.5 | 15.1 | 17.1 | 16.1 | ||
| tim > KK101716 | 16 | 0 | 24.8 | 101.1 | 4.9 | 1.1 | 12.1 | 17.6 | 14.8 | ||
| Itpr | 1,063 | pdf > itprRNAi#1 | 36 | 11 | 24.2 | 51.7 | 4.0 | 0.8 | 18.9 | 18.1 | 19.4 |
| tim > itprRNAi#1 | 20 | 50 | 24.4 | 14.7 | 2.8 | 0.9 | 9.0 | 5.6 | 9.7 |
*All genotypes include UAS-dcr2 if it is not mentioned (UAS = Upstream Activating Sequence). CG no.: coding gene number in fly genome; AR: arrhythmic; pwr: power of rhythmicity by chi-square periodogram; wid: width of rhythmicity peak by chi-square periodogram; ACT: averaged locomotor activity level.
†N = flies alive at the end of the testing period.
‡In this genotype, 69% of flies died during the behavioral experiment.
** SNR = Signal to Noise Ratio