Table 2. Specific MRM parameters and retention times (RT) for each analyte and internal standard (IS).

Analyte	MRM transition	RT (min)	DP	CE	CXP
OHCbl	665.3 → 636.5a	3.60	80	25	14
	665.3 →147.2b	3.60	80	60	8
CNCbl	678.3 → 147.3a	3.80	85	60	8
	678.3 → 359.2b	3.80	85	32	15
AdoCbl	790.6 → 665.5a	3.85	80	30	15
	790.6 → 147.3b	3.85	80	80	8
MeCbl	672.8 → 665.4a	3.98	75	25	16
	672.8 → 147.1b	3.98	75	66	6
(IS)	682.0 → 154.2	3.80	85	60	8

^a: quantifier transition,

^b: qualifier transition.