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. 2022 Jun 7;17(6):e0269281. doi: 10.1371/journal.pone.0269281

Fig 1. The Arc constructs used in this study.

Fig 1

FLrArc-7A is full-length rArc (residues 1–397) with residues 113–119 mutated to Ala to hinder capsid formation [18]. hArc-CTD contains residues 207–362. Not shown is the N-terminal 6xHis-tag and the linker containing the TEV site. 2rNT is the NTD of rArc (residues 24–137) with the poly-Ala mutation. The domain was insoluble in the absence of its fusion partner, maltose binding protein (MBP). FLhArc corresponds to FLrArc-7A, but with the human Arc sequence without any mutations.