Fig 2. Anti-Arc nanobodies.
A Sequence alignment reveals the location of the three CDRs, highlighting the long CDR3 of NbArc-H11. Sequences are coloured by conservation, and Cys24 and Cys92 (Kabat numbering) are highlighted in yellow. The alignment was produced using Clustal Omega [71] and JalView [72]. B Determination of NbArc Tm using DSF (N = 3). The Tm of NbArc-D4 could not be determined. C 1.42-Å crystal structure of NbArc-E5. Colouring of CDRs is the same as in panel A. D The conserved central disulphide bond, formed by oxidation Cys24 and Cys97, packs against Trp38 and is partially reduced. Electron density is shown as a blue mesh at 1.5σ.