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. 2022 Jun 7;17(6):e0269281. doi: 10.1371/journal.pone.0269281

Fig 6. Rudimental analysis of Nb association with hArc capsid fraction.

Fig 6

A Analytical SEC of capsid hArc-Nb complexes. 2 nmol of Arc capsids were mixed with a 1.5-fold molar excess of Nbs and run on a Superdex 200 Increase 10/300 GL in PBS buffer. Noted is the presumably dimeric peak present in some Nb complexes, with an elution volume corresponding to that of the FLrArc-7A complexes. NbArc-E5 was accidentally mixed with 4 nmol of hArc, resulting in the large void peak and lack of Nb excess peak. B SDS-PAGE analysis of SEC fractions, demonstrating that Nbs co-elute with the capsids. C Negative staining TEM images of capsid hArc at 150,000x magnification with NbArc-H11 (left) and without Nb (right). Arrows point at capsids, and a magnified view of selected capsids is shown.