Table 1. Thermal stability and thermodynamics of Nb binding to FLrArc-7A.

Nb	ΔTm (°C)	Number of sites	Kd (nM)	ΔH (kJ mol-1)	-TΔS (kJ mol-1)	ΔG (kJ mol-1)
B12	+1.1	1.040±0.010	7.34±2.97	-36.00±0.70	-10.11	-46.11
B5	+8.7	0.875±0.003	0.66±0.29	-41.05±0.29	-10.99	-52.04
C11	+13.4	0.815±0.002	3.49±0.85	-55.62±0.43	7.97	-47.64
D4	N/D	0.845±0.003	1.19±0.27	-98.49±0.64	48.37	-50.12
E5	+5.2	0.967±0.003	2.34±0.63	-110.00±1.00	61.27	-48.73
H11	+24.0	0.892±0.003	1.12±0.45	-107.57±0.51	56.28	-51.29

ΔT_m was measured using DSF (Fig 4A). Thermodynamics of binding were derived from ITC (Fig 4B). N/D: Not determined.