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. 2022 May 24;10:816275. doi: 10.3389/fbioe.2022.816275

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Copyright © 2022 Chouquet, Pinto, Hennicke, Ling, Bally, Schwaigerlehner, Thielens, Kunert and Reiser.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Size exclusion chromatography coupled to multi-angle laser light scattering (SEC-MALLS) and analytical ultracentrifugation (AUC) analysis of purified IgM617 samples from HEK293F expression. The left panels show the elution profiles of the purified (A) IgM617-HLJ and (B) IgM617-HL monitored by the excess refractive index (right ordinate axis) and the molecular weight as bold line (left ordinate axis) derived from MALLS, refractometry, and UV-Vis measurements. The estimated average molecular weights are indicated on the graphs. The right panels show the sedimentation distributions of (C) purified IgM617-HLJ and (D) IgM617-HL. Calculated and corrected sedimentation coefficients S_20,W are obtained as described in Materials and Methods and are indicated on the graphs.