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. 2022 May 24;10:816275. doi: 10.3389/fbioe.2022.816275

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Copyright © 2022 Chouquet, Pinto, Hennicke, Ling, Bally, Schwaigerlehner, Thielens, Kunert and Reiser.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Complement activation by the different purified IgM constructs expressed in HEK293F. Recombinant IgMs and IgMs purified from plasma were coated on microplate wells and incubated with either normal human serum (NHS), C1q-depleted NHS (NHSΔ), or reconstituted NHS (NHSΔ + C1q). C1 activity was monitored via C4b deposition. Reported values are average of replicated (2–4) normalized experiments (100% = IgM plasma/NHS). Errors are obtained as standard deviation between independent replicates.