. 2022 May 19;12(11):6570–6577. doi: 10.1021/acscatal.2c01184

Table 2. Michaelis–Menten Kinetic Constants of Selected Fluorinases^a.

fluorinase	K_M^SAM (μM)	k_cat (min^–1)	k_cat/K_M^SAM (mM^–1 min^–1)
FlA^MA37	4.42 ± 0.58	0.16 ± 0.01	36.36 ± 4.82
FlA^Sxin	3.76 ± 0.15	0.22 ± 0.01	58.63 ± 2.63
FlA^SAJ15	9.62 ± 1.43	0.34 ± 0.01	35.81 ± 5.43
FlA^PtaU1	6.99 ± 1.06	0.41 ± 0.01	57.54 ± 8.85

Assays conducted in 50 mM HEPES, pH = 7.8, with 75 mM KF and varying SAM concentrations incubated at 37 °C. Average and standard deviations are given for triplicate independent measurements.

Table 2. Michaelis–Menten Kinetic Constants of Selected Fluorinasesa.

Table 2. Michaelis–Menten Kinetic Constants of Selected Fluorinases^a.