Appendix 1—table 1. List of fluorescent antibiotic derivatives (obtained by linking the parental antibiotic to nitrobenzoxadiazole, NBD, see Methods), the bacterial compartment where their target is located, their molecular weight (MW) after linkage to NBD, their partition coefficient (logP), their measured minimum inhibitory concentration (MIC) against E. coli BW25113, and the fold-change compared to the MIC measured for each corresponding parental antibiotic (see Methods).
MIC data were collated from biological triplicate.
| |||||
|---|---|---|---|---|---|
| Antibiotic probe | Compartment | MW (g/mol) | logP |
MIC
(μg/mL) |
Fold change |
| Polymyxin B-NBD | Membrane | 1,449 | –2.5 | 1 | 1 |
| Octapeptin-NBD | Membrane | 1,304 | –0.4 | 4 | 1 |
| Tachyplesin-NBD | Membrane | 2,523 | –2.7 | 1 | 1 |
| Vancomycin-NBD | Cell wall | 1,650 | –2.6 | >192 | 1 |
| Linezolid-NBD | Cytoplasm | 638 | 0.7 | 134 | 1 |
| Roxithromycin-NBD | Cytoplasm | 1,064 | 3.1 | 192 | 3 |
| Ciprofloxacin-NBD | Cytoplasm | 633 | –1.1 | 8 | 256 |
| Trimethoprim-NBD | Cytoplasm | 577 | 0.9 | 64 | 64 |