Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Anti-Piwi1 (rabbit polyclonal) | https://doi.org/10.1126/science.aay6782 | N/A | IF (1:2000) |
| Antibody | Anti-Piwi2 (Guinea pig polyclonal) | In-house | N/A | IF (1:500) |
| Antibody | Anti-acetylated tubulin (mouse monoclonal) | Sigma-Aldrich | Ca# T7451 | IF (1:1000) |
| Antibody | Anti-RFamide (mouse polyclonal) | https://doi.org/10.1007/s004270050181 | IF (1:1000) | |
| Antibody | Anti-GLWamide (rabbit polyclonal) | https://doi.org/10.1007/s004270050181 | IF (1:1000) | |
| Antibody | Anti-Ncol1 (rabbit polyclonal) |
https://doi.org/10.1371/journal.pone.0022725.g001
|
IF (1:500) | |
| Antibody | Anti-Ncol3 (guinea pig polyclonal) | https://doi.org/10.1371/journal.pone.0022725.g001 | IF (1:500) | |
| Antibody | Anti-GFP (rabbit polyclonal) | Santa Cruz | Ca# 8334 | IF (1:1000) |
| Antibody | Anti-RFP (rat polyclonal) | Chromotek | Ca# 5F8 | IF (1:1000) |
| Strain, strain background (Hydractinia symbiolongicarpus) | 293-10 wild type animals | This paper | N/A | Materials and methods |
| Strain, strain background (Hydractinia symbiolongicarpus) | SoxB1::tdTomato line | This paper | N/A | Materials and methods |
| Strain, strain background (Hydractinia symbiolongicarpus) | SoxB2::GFP line | This paper | N/A | Materials and methods |
| Strain, strain background (Hydractinia symbiolongicarpus) | RFamide::GFP line | This paper | N/A | Materials and methods |
| Strain, strain background (Hydractinia symbiolongicarpus) | RFamide::SoxB1::GFP | This paper | N/A | Materials and methods |