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. 2022 May 18;119(21):e2200022119. doi: 10.1073/pnas.2200022119

Fig. 5.

Fig. 5.

NO induces DNA damage via a p38/DNMT1/LINE-1 mechanism. (A) Immunoblot of γ-H2AX expression in DETANO-treated parental or p38α-inactive mutant MCF10A cells compared with vehicle controls. Graph shows normalized mean γ-H2AX expression as fold change (fc) of vehicle controls (±SEM; n = 3). Significance was calculated by two-way ANOVA with Šídák's multiple comparisons test. (B) Immunoblot of γ-H2AX expression in control or Myc-DNMT1 overexpressing HEK293 cells treated with vehicle or DETANO for 24 h. Graph shows normalized mean γ-H2AX densitometric ratios as fold change of control (±SEM; n = 3). Significance was calculated by one-way ANOVA with Dunnett's multiple comparisons test. (C) Immunoblot of γ-H2AX expression in MDA-MB-231 cells transfected with either control or LINE-1 silencing RNA and cultured with vehicle or DETANO for 24 h. Graph shows the mean densitometric analyses of normalized γ-H2AX expression (±SEM; n = 3). Significance was calculated by two-way ANOVA with Tukey's multiple comparisons test. siRNA, small interfering RNA.