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. 2022 May 19;119(21):e2119483119. doi: 10.1073/pnas.2119483119

Fig. 3.

Fig. 3.

Heterozygous expression of CXCR4R334X abrogates CXCL12-mediated CXCR4 dynamics and nanoclustering. SPT analysis of CXCR4-AcGFP in JKX4−/−-X4 and JKX4−/−-R334X+-X4 cells on fibronectin (FN) or FN+CXCL12-coated coverslips (1,176 particles in 60 cells on FN; 3,037 in 66 cells on FN+CXCL12 in JK-X4 cells; 867 in 72 cells on FN; 1,525 in 75 cells on FN+CXCL12 in JKP ×4−/−R334X+-X4 cells; n = 3). (A) Diffusion coefficients (D1–4) of mobile single trajectories, with median (black line) corresponding to JK-X4 and JKX4−/−-R334X+X4 cells (n.s., not significant; ****P ≤ 0.0001). (B) Frequency of CXCR4-AcGFP particles containing the same number of receptors [monomers plus dimers (≤2) or nanoclusters (≥3) in JK and JKX4−/−R334X+ cells, calculated from MSI values of each particle as compared with the MSI value of monomeric CD86-AcGFP.