Fig. 4.

H3K9MTs repress Enz-induced activation of retroelements. (A) RT-qPCR analyses showing the induction of the indicated transcripts from SINE, LINE, simple repeat, and ERV families in LNCaP cells treated with Enz (10 µM) for 14, 56, or 380 d, as compared with the respective Veh-treated controls. Endpoints were compared using a one-way ANOVA. (B) Enz/Veh fold-change induction levels of the indicated repeat element transcripts in LNCaP cells stably overexpressing the EV or H3K9-methyltransferases (OE), following treatment with Enz (5 µM) or vehicle for 40 d. P values were calculated using two-way ANOVA; error bars represent SD. Results are a representative of two biological repeats performed in triplicate. ****P < 0.001.