Fig. 3.
MERS-nsp15H231A/ΔNS4a and MERS-nsp15H231A/NS4bH182R induce IFN/ISG expression in A549DPP4 cells. A549DPP4 cells were mock infected or infected in triplicate at MOI = 5. Total RNA was harvested at 24 and 48 hpi, and expression of IFNL1, IFNB, IFIH1, OAS2, and IFIT1 mRNAs was quantified by qRT-PCR and expressed as fold change over mock infected using the 2−Δ(ΔCT) formula. Data are displayed as means ± SD. Data are from one representative of three independent experiments. Statistical significance compared to WT MERS-CoV was calculated by two-way ANOVA: *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001. Data that were not statistically significant are not labeled.