Fig. 4.
RNase L and PKR are activated in the absence of EndoU activity combined with loss of accessory proteins during MERS-CoV infection. (A) A549DPP4 cells were mock infected or infected at MOI = 5, and total cellular RNA was harvested at 24 and 48 hpi. The rRNA degradation was assessed on an Agilent Bioanalyzer. 28S and 18S rRNA positions are indicated. Data are from one representative of three independent experiments. (B) A549DPP4 cells were mock infected or infected at MOI = 5 and cell lysates were harvested at 24 and 48 hpi. Proteins were separated by SDS/PAGE and immunoblotted with antibodies against phosphorylated PKR (p-PKR), PKR, phosphorylated eIF2α (p-eIF2α), eIF2α, MERS-CoV nucleocapsid (N), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Data are from one representative of four (24 hpi) or two (48 hpi) independent experiments.