TABLE 2.
Sequence of 24-mer oligonucleotide SCAR primers derived from RAPD markers that distinguish T. hamatum 382 from other T. hamatum isolates and Trichoderma spp.
| SCAR markersa | Primerb | Sequencec |
|---|---|---|
| SCH19588 | OPH19-F | CTGACCAGCCTGTTAAAATCAT |
| OPH19-R | CTGACCAGCCCAAAGACTCCC | |
| SCE16347 | OPE16-F | GGTGACTGTGGCCTTGTTTGCATA |
| OPE16-R | GGTGACTGTGAATGGCGAAGCTAC | |
| SCH20624 | OPH20-F | GGGAGACATCGCATCTGCATGTAA |
| OPH20-R | GGGAGACATCAACGATGATTCAGC |
a
Subscript number refers to size of amplified product from genomic DNA of T. hamatum 382.
b
Letters H and E refer to the kit, and numbers 19, 16, and 20 refer to the random primer number from Operon Technologies. The letters F and R refer to forward and reverse primers from both ends of the RAPD fragment cloned.
c
Sequences of random primers used in RAPD analysis are underlined.