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. 2022 May 16;119(21):e2015576119. doi: 10.1073/pnas.2015576119

Fig. 1.

Fig. 1.

ascr#10 increases proliferation of germline precursors. (A and B) Representative images of DAPI-stained progenitor zones in day 5 N2 hermaphrodites aged on (A) control or (B) ascr#10 plates. (Scale bars, 10 μm.) Proximal are to the left, and distal are to the right. Dashed vertical lines delimit proximal boundaries of progenitor zones, as defined by Crittenden et al. (70). Notice the higher number of mitotic figures (arrowhead) in B. (C and D) Experimental scheme and results are shown for (C) number of GPCs in N2 hermaphrodites reared on or off ascr#10 from egg lay to indicated stage (in hours after hatching) and (D) number of GPCs in N2 hermaphrodites reared on or off ascr#10 from 48 h following release from L1 arrest to indicated stage. In C and D, each dot is the number of GPCs in one individual; horizontal bars represent means. (E) Schematic representation of one arm of hermaphrodite gonad showing the GPC population and mitotically dividing cells (magenta). (F) Representative DAPI-stained progenitor zones from hermaphrodites aged on or off ascr#10. pH3-positive cells are shown in magenta. (Scale bars, 10 μm.) (G and H) Mitotic events in gonads of (G) larvae and young adults exposed to ascr#10 since early embryogenesis and (H) adult hermaphrodites exposed to ascr#10 since early adulthood. *P < 0.05; **P < 0.01; ***P < 0.001; n.s., not significant. See Table S1 for primary data and details of statistical analyses.